作者：吕立夏主编
页数：96页
出版社：同济大学出版社
出版日期：2017
ISBN：9787560871219
电子书格式：pdf/epub/txt
内容简介
吕立夏主编的《生命科学综合实验(英文版)》将进一步指导读者学习和实践基本的和前沿的细胞学和生物化学实验技术。本教程主要适用于医学专业留学生，同时也可供学习专业英语的医学生使用。
本书特色
生物化学和细胞生物学是生命科学的重要基础学科 其基本实验技术和方法更是现代生命科学研究的基石。本教材的目的是使医学院校本科学生初步掌握生物化学和细胞生物学基本实验原理、方法和技术 为将来的科学研究奠定基础。
目录
Preface
Part 1 Biochemistry Experiment
Experiment 1 Use of the Spectrophotometer & Determination of Protein Concentration
Experiment 2 Chromatography
Experiment 2 (1) Using Gel Filtration Column Chromatography to Separate Hemoglobin and Nucleoprotamine
Experiment 2 (2) Separating Amino Acids Mixture by Lon-Exchange Chromatography
Experiment 3 Determination of Km of Alkaline Phnsphatase
Experiment 4 Centrifugation Technique–Adaptive Immune Response: Isolation and Identification of Lymphocyte
Experiment 4 (1) The Separation of Monocytes by Density Gradient Centrifugation
Experiment 4 (2) Lymphocyte Identification–Counting the Number of B Lymphocyte
Experiment 5 Polyacrylamide Gel Electrophoresis of Proteins
Experiment 6 Effect of Hormone on Blood Sugar and Lipoprotein of Normal and Diabetic rat
Experiment 7 Polymorphism Analysis of ACE Gene by Extracting Genome DNA of Buccal Epithelial Cells
Part 2 Cell Biology Experiment
Experiment 8 Optical Microscope and Cell Cycle
Experiment 9 Cell Culture and Ceil Fusion
Experiment 10 Primary Culture and Directional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
Reference
Part 1 Biochemistry Experiment
Experiment 1 Use of the Spectrophotometer & Determination of Protein Concentration
Experiment 2 Chromatography
Experiment 2 (1) Using Gel Filtration Column Chromatography to Separate Hemoglobin and Nucleoprotamine
Experiment 2 (2) Separating Amino Acids Mixture by Lon-Exchange Chromatography
Experiment 3 Determination of Km of Alkaline Phnsphatase
Experiment 4 Centrifugation Technique–Adaptive Immune Response: Isolation and Identification of Lymphocyte
Experiment 4 (1) The Separation of Monocytes by Density Gradient Centrifugation
Experiment 4 (2) Lymphocyte Identification–Counting the Number of B Lymphocyte
Experiment 5 Polyacrylamide Gel Electrophoresis of Proteins
Experiment 6 Effect of Hormone on Blood Sugar and Lipoprotein of Normal and Diabetic rat
Experiment 7 Polymorphism Analysis of ACE Gene by Extracting Genome DNA of Buccal Epithelial Cells
Part 2 Cell Biology Experiment
Experiment 8 Optical Microscope and Cell Cycle
Experiment 9 Cell Culture and Ceil Fusion
Experiment 10 Primary Culture and Directional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
Reference
