作者：张雁
页数：190
出版社：中山大学出版社
出版日期：2020
ISBN：9787306068989
电子书格式：pdf/epub/txt
内容简介
本教材为全英文教材，可以使学生在完成较为复杂的综合实验过程中，培养独立获取知识、解决问题的实践技能和综合素质，让学生养成良好的深层次、进取式学习的好习惯。教材的知识目标是要学生掌握实验原理，掌握基本实验技能；能力目标是要让学生获得提出生命科学真问题的能力、分析点评结果的能力、解决复杂问题的能力，自主学习和深度学习的能力；情感目标是要让学生懂得团队合作，关爱他人，遵守学术规范，敬畏生命和规则。
作者简介
张雁，中山大学生命科学学院教授，博士生导师，从事肿瘤与干细胞研究。生物科学综合实验、新生研讨课和生物医学基础的课程负责人。
目录
Chapter 1 DNA Isolation
Section 1 DNA in a Bottle
Section 2 Isolation and Purification of Genomic DNA from Cheek Cells
Section 3 DNA Isolation from Chicken Embryonic Allantoic Fluid
Section 4 Know More
Chapter 2 DNA Analysis
Section 1 Do You Have Jumping Alu in Your Genome?
Section 2 Boy or Girl?
Section 3 Know More
Chapter 3 RNA Extraction and RT-PCR
Section 1 RNA Extraction and Purification
Section 2 RT-PCR
Section 3 TA Cloning
Chapter 4 Bacterial Transformation and Plasrnid Purification
Section 1 Bacterial Transformation and Culture
Section 2 Colony PCR Screening for the Reconstructed Plasmids
Section 3 Purification of Plasmid DNA
Section 4 Know More
Chapter 5 Protein Extraction
Section 1 Preparation of Protein
Section 2 Know More
Chapter 6 Protein Analysis
Section 1 SDS-PAGE and Western Blotting
Section 2 Know More
Chapter 7 Animal Cell Culture
Section 1 Subculturing Cells
Section 2 Cryopreservation
Section 3 Recovery of Cryopreserved Cells
Section 4 Know More
Chapter 8 Cell Transfection
Section 1 Transient Transfection
Section 2 Detecting Transfection Efficiency by Flow Cytometry
Chapter 9 Cell Contamination
Section 1 Types of Cell Contamination
Section 2 Detection of Contamination
Section 3 Removal of Contamination
Section 4 Know More
Chapter 10 Genome Editing by CRISPR/Cas9
Section 1 CRISPR/Cas9 System
Section 2 Cloning a gRNA into a Vector
Section 3 Purification of Cas9/sgRNA Coexpression Vectors
Section 4 Cotransfection of Cas9/sgRNA Coexpression Vectors into the Cells
Section 5 PCR Analysis of Targeted Deletion in the Genome
Section 6 Know More
Chapter 11 Biosafety in Laboratory
Section 1 General Laboratory Safety
Section 2 Biosafety
Chapter 12 The Laboratory Report
Appendix
Section 1 Experimental Instruments
Section 2 Preparation of Commonly–Used Solutions
Section 1 DNA in a Bottle
Section 2 Isolation and Purification of Genomic DNA from Cheek Cells
Section 3 DNA Isolation from Chicken Embryonic Allantoic Fluid
Section 4 Know More
Chapter 2 DNA Analysis
Section 1 Do You Have Jumping Alu in Your Genome?
Section 2 Boy or Girl?
Section 3 Know More
Chapter 3 RNA Extraction and RT-PCR
Section 1 RNA Extraction and Purification
Section 2 RT-PCR
Section 3 TA Cloning
Chapter 4 Bacterial Transformation and Plasrnid Purification
Section 1 Bacterial Transformation and Culture
Section 2 Colony PCR Screening for the Reconstructed Plasmids
Section 3 Purification of Plasmid DNA
Section 4 Know More
Chapter 5 Protein Extraction
Section 1 Preparation of Protein
Section 2 Know More
Chapter 6 Protein Analysis
Section 1 SDS-PAGE and Western Blotting
Section 2 Know More
Chapter 7 Animal Cell Culture
Section 1 Subculturing Cells
Section 2 Cryopreservation
Section 3 Recovery of Cryopreserved Cells
Section 4 Know More
Chapter 8 Cell Transfection
Section 1 Transient Transfection
Section 2 Detecting Transfection Efficiency by Flow Cytometry
Chapter 9 Cell Contamination
Section 1 Types of Cell Contamination
Section 2 Detection of Contamination
Section 3 Removal of Contamination
Section 4 Know More
Chapter 10 Genome Editing by CRISPR/Cas9
Section 1 CRISPR/Cas9 System
Section 2 Cloning a gRNA into a Vector
Section 3 Purification of Cas9/sgRNA Coexpression Vectors
Section 4 Cotransfection of Cas9/sgRNA Coexpression Vectors into the Cells
Section 5 PCR Analysis of Targeted Deletion in the Genome
Section 6 Know More
Chapter 11 Biosafety in Laboratory
Section 1 General Laboratory Safety
Section 2 Biosafety
Chapter 12 The Laboratory Report
Appendix
Section 1 Experimental Instruments
Section 2 Preparation of Commonly–Used Solutions
