梁宋平教授文集-蛋白质与活性多肽探索

第一部分 蛋白质化学与蛋白质组学学术论文 8-（6-氨基己基）-氨基-5-磷酸腺苷Sepharose 4B亲和吸附剂的制备与应用 大熊猫乳酸脱氢酶同工酶MA的纯化与某些性质的研究 The primary structure of the lactate dehydrogenase isozyme Ma from giant panda A comparison of the primary structures of lactate dehydrogenase isozymes M4 from giant panda，red panda．black bear and dog Covalent immobilization of proteins and peptides for solid—phase sequencing using prepacked capiHary columns Single．step electroelution of proteins from SDS—polyacrylamide gels and immobilization on diisothiocyanate—glass beads in prepacked capillary columns for solid—phase microsequencing Assignment of the three disulfide bridges of huwentoxin-I，a neurotoxin from the spider Selenocosmia huwena Chemical carboxy-terminal sequence analysis of peptides using acetyl isothiocyanate 世纪之交的蛋白质序列测定技术 Assignment of the three disulfide bonds of Selenocosmia huwena lectin I from the venom of spider Selenocosmia huwena Indentification of venom proteins of spider S．huwena on two-dimensional electrophoresis gel by N—terminal microsequencing and mass spectrometric peptide mapping Chemical carboxyl．terminal sequence analysis of peptides and proteins using tribenzylsilyl isothiocyanate Assignment of the disulfide bonds of huwentoxin—II by Edman degradation sequencing and stepwise thiol modification C-terminal sequence analysis of peptides using triphenylgermanyl isothiocyanate De nolo sequencing of tryptic peptides sulfonated by 4-sulfophenyl isothiocyanate for unambiguous protein identification using post-source decay matrix．．assisted laser desorption／ionization mass spectrometry Proteome comparative analysis of gynogenetic haploid and diploid embryos of goldfish(Carassius auratus) Proteomic analysis of mouse liver plasma membrane：use of differential extraction to enrich hydrophobic membrane proteins Integration of a two—phase partition method into proteomics research on rat liver plasma membrane proteins 第二部分 蜘蛛活性多肽研究学术论文 Properties and amino acid sequence of huwentoxin-I，a neurotoxin purified from the venom of the Chinese bird spider Selenocosmia huwena 虎纹捕鸟蛛毒的生物学活性鉴定 蜘蛛肽类神经毒素研究进展 A lectin—like peptide isolated from the venom of the Chinese bird spider Selenocosmia huwena Proton nuclear magnetic resonance studies on huwentoxin—I from the venom of the spider Selenocosmia huwena：2．ThFee．dimensional structure in solution DEPD：a novel database for differentially expressed proteins Solid’phase synthesis of huwentoxin—I and its structure and bioactivity analysis Oxidative folding of reduced and denatured huwentoxin．I Three—dimensional structure of Selenocosmia huwena lectin—I(SHL．I)from the venom of the spider Selenocosmia huwena by 2D-NMR Purification and characterization of huwentoxin—II，a neurotoxic peptide from the venom of the Chinese bird spider Selenocosmia huwena 我国南方捕鸟蛛一新种的生物化学鉴定(蜘蛛目，捕鸟蛛科) The presynaptic activity of huwentoxin—I，a neurotoxin from the venom of the Chinese bird spider Selenocosmia huwena The effect of Huwentoxin—I on Ca2+channels in differentiated NG108-15 cells，a patch—clamp study Function and solution structure of huwentoxin—IV，a potent neuronal tetrodotoxin(TI’X)一sensitive sodium channel antagonist from Chinese bird spider Selenocosmi huwena The structure of spider toxin huwentoxin—11 with unique disulfide linkage：evidence for structural evolution cDNA sequence analysis of seven peptide toxins from the spider Selenocosmia huwena’ Purification and characterization of raventoxin．I and raventoxin—III，two neurotoxic peptides from the venom of the spider Macrothele raveni Structure—activity relationships of hainantoxin—IV and structure determination of active and inactive sodium channel blockers An overview of peptide toxins from the venom of the Chinese bird spider Selenocosmia huwena Wang[=Ornithoctonus huwena(Wang)] Jingzhaotoxin—III，a novel spider toxin inhibiting activation of voltage—gated sodium channel in rat cardiac myocytes Antinociceptive effects of intrathecally administered huwentoxin—I，a selective N—type calcium channel blocker．in the formalin test in conscious rats Jingzhaotoxin-I，a novel spider neurotoxin preferentially inhibiting cardiac sodium channel inactivation Function and solution structure of Huwentoxin—X，a specific blocker of N-type calcium channels．from the Chinese bird spider Ornithoctonus huwena Solution structure and functional characterization of Jingzhaotoxin—XI：A novel gating modifier of both potassium and sodium channels Discovery of a distinct superfamily of Kunitz—type toxin(KTI”)from Tarantulas Proteome and peptidome profiling of spider venoms 第三部分 其他散文与短文 寄语即将毕业的同学们 从伦敦到诺丁汉——访英随笔之一 阿希武德教授——访英随笔之二 校园绿地与RAMP——访英随笔之三 诺丁汉大学生命科学系——访英随笔之四 从数字看诺丁汉大学——访英随笔之五 电子邮件(E—Mail)与环球信息网(WWW)——访英随笔之六 神经生物学研究室——访英随笔之七 揭开生命科学的奥秘 心中的铭记——缅怀恩师张龙翔教授 开学感赋——与九七级研究生共勉 《生命科学研究》发刊词 登攀桥赤子亭记 《生命科学院开发应用科研成果汇编》前言 关于学位论文实验——与新入学生物化学研究生的开学笔谈 《生物化学与分子生物学实验教程》前言 生命科学对教育科学的启示——2005年在湖南师范大学研究生“麓山论坛”上的讲座 蛛海拾贝——在第六届全国生物毒素研讨会上所作学术报告的结束语 与2006级生命科学学院研究生共勉 致敬爱的谢锦云老师 在2008研究室工作会议上的结语 一位美国教授的中国情结——记波士顿大学教授，湖南师范大学名誉教授R．A．Lauursen 附录 后记