作者：欧田苗
页数：134
出版社：中山大学出版社
出版日期：2022
ISBN：9787306067999
电子书格式：PDF
PDF下载地址
内容简介
本书为全英文教材，作为高等教育出版社出版的《生物化学与基础分子生物学实验》（2019年出版）的补充教材。本书的主要编写目的是希望强调以下几点：（1）使学生能够掌握规范的实验技能；（2）使学生能够初步具备生物化学学科的科学思维及素养，包括合理的实验设计能力、严谨的数据处理能力和分析及解决问题的能力等；（3）使学生掌握生物化学与分子生物学实验中的专业词汇及常用表述方式。
目录
Part I Theory and General Techniques
Chapter 1 Introduction
Chapter 2 General Laboratory Procedures
Chapter 3 Extraction, Purification, and Concentration of Biomolecules
Chapter 4 Purification and Identification of Biomolecules by Chromatography
Chapter 5 Characterization of Biornolecules by Electrophoresis
Part II Experiments
Experiment 1 Properties of Proteins
Experiment 2 Quantitative Determination of Protein
Experiment 3 Extraction and Purification of Tyrosinase from Potato and Determination of the Enzyme Activity
Experiment 4 Determination of the Michaelis Constant for Tyrosinase and Effects that Affect the Activity
Experiment 5 Staining and Identification of Active Superoxide Dismutase
Experiment 6 Preparation of E.Coli Competent Cells and Introduction DNA into Host Cells
Experiment 7 Extraction and Quantitative Analysis of Plasmid DNA via Alkaline Lysis Method
Experiment 8 Identification of Transformation Products via Polymerase Chain Reaction and Restriction Endonuclease Digestion
Experiment 9 Extraction and Identification of Total RNA from Animal Tissues and Cells
Experiment 10 Reverse Transcription-polymerase Chain Reaction
Chapter 1 Introduction
Chapter 2 General Laboratory Procedures
Chapter 3 Extraction, Purification, and Concentration of Biomolecules
Chapter 4 Purification and Identification of Biomolecules by Chromatography
Chapter 5 Characterization of Biornolecules by Electrophoresis
Part II Experiments
Experiment 1 Properties of Proteins
Experiment 2 Quantitative Determination of Protein
Experiment 3 Extraction and Purification of Tyrosinase from Potato and Determination of the Enzyme Activity
Experiment 4 Determination of the Michaelis Constant for Tyrosinase and Effects that Affect the Activity
Experiment 5 Staining and Identification of Active Superoxide Dismutase
Experiment 6 Preparation of E.Coli Competent Cells and Introduction DNA into Host Cells
Experiment 7 Extraction and Quantitative Analysis of Plasmid DNA via Alkaline Lysis Method
Experiment 8 Identification of Transformation Products via Polymerase Chain Reaction and Restriction Endonuclease Digestion
Experiment 9 Extraction and Identification of Total RNA from Animal Tissues and Cells
Experiment 10 Reverse Transcription-polymerase Chain Reaction